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Anti-PFK1-7 | Phosphofructokinase 1-7
Product no: AS23 4914
AS23 4914 | Clonality: Polyclonal |Host: Rabbit | Reactivity: Arabidopsis thaliana
329
€
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- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PFK1-7 protein sequences, UniProt: Q9M0F9 , Q9FIK0,Q94AA4, Q9FKG3 Q8VYN6 Q9M076 Q9C5J7 TAIR: At4g29220, At5g47810, At4g26270, At5g61580, At2g22480, At4g32840, At5g56630 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 - 1: 2000 (WB) Expected | apparent MW: 51.9 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica napus (PFK1, PFK3, PFK5, PFK6, PFK7),
Nicotiana tabacum (PFK3, PFK4, PFK5, PFK6)
Pisum sativum (PFK3, PFK4, PFK5, PFK7,PFK6)
Solanum lycopersicum ( PFK3, PFK4m PFK5, PFK6
Solanum tuberosum ( PFK3, PFK4, PFK5, PFK6)
Arachis hypogaea, Brachypodium distachyon, Brassica napus, Cannabis sativa, Hordeum vulgare, Malus domestica, Manihot esculenta, Medicago truncatula, Nicotiana tabacu,Oryza sativa, Saccharum sp., Theobroma cacao, Triticum sp, ,Sorghum bicolor, Zea mays, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
20 ug of Arabidopsis thaliana whole leaf extract
20 ug of Arabidopsis thaliana mutant described above the picture MW markers are marked on the left side of each membrane
20 µg/well of total protein extracted freshly from leaf extracts of Arabidopsis thaliana wildtype and mutants. Exact buffer components were: 50 mM Hepes-KOH (pH 6.8), 5 mM Mg-acetate, 15 % Glycerin, 1 mM EDTA, 1 mM EGTA, 5 mM ß-Mercaptoethanol, 0.1 mM Pefabloc Proteinase-inhibitor and denatured with 1 x Laemmli-buffer (62.5 mM Tris-HCl (pH 6.8), 2 % SDS, 10 % Glycerin, 5 % ß-Mercaptoethanol, 0.001 % Bromphenolblue) at 98 °C / 2 mins. Samples were separated on 10 % SDS-PAGE and blotted for 1 h PVDF (pore size of 0.45 µm), using: wet transfer in the cold. Blot was blocked with 5 % nonfat milk 4°C / ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1 h/RT with agitation in TBS-T. The antibody solution was decante, andd the blot was rinsed briefly, then washed once 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 25 000 in for 1 h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent. Exposure time was 3 minutes.Courtesy of phd student Alina Johanna Hieber, University of Bayreuth, Bayreuth, Germany
- Background
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Background: PFK (Phosphofructokinase) is the enzyme involved in the first committing step of glycolysis. It catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP.
Alternative name: Phosphohexokinase - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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