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Anti-HXK1 | Hexokinase 1
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana hexokinase-1, UniProt: Q42525, TAIR:AT4G29130
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 53 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Pisum sativum
Predicted reactivity: Actinidia chinensis, Arabis alpina, Brassica napus, Camellia sinensis, Capsella rubella, Coffea canephora, Cucumis sativus, Dimocarpus longan, Eucalyptus grandis, Glycine max, Gossypium raimondii, Jatropha curcas, Malus domestica, Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: Chlamydomonas reinhardtii, Saccharomyces cerevisiae
- Application Examples
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application example
ca. 4 µg and 10 µg of total protein (outer envelop of chloroplasts) from Pisum sativum leaves extracted with 20m M Mops, 13 mM Tris, 0.1 mM MgCl2 330 mM Sorbit 0.02% BSA (stored in NaPO4) were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 1% milk 1x TBS-T for 3x10 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody(goat anti-rabbit IgG, HRP conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk1xTBS-T for 1h at RT with agitation. The blot was washed as above and developed for 1 min with combination of 100 mM Tris-HCL pH 8.5, 1%Luminol, 0.44% Coomaric Acid and 100 mM Tris-HCl pH 8.5, 0.018% H2O2 (1mL of each Solution, selfmade). Exposure time was 60 seconds.
Courtesy of Bettina Mathes, Ludwig Maximilians University Munich, Germany - Background
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Background: HXK1 (Hexokinase 1) is an enzyme of plant glucose-signaling network which functions as a glucose sensor.
Alternative name: Protein GLUCOSE INSENSITIVE 2. - Product Citations
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Selected references: Gil et al. (2017) ZEITLUPE Contributes to a Thermoresponsive Protein Quality Control System in Arabidopsis. PlantCell. 2017 Nov;29(11):2882-2894. doi: 10.1105/tpc.17.00612. - Protocols
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- Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS12 2119 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. platanoides, A. thaliana, C.australis R.Br., L. longiflorum. M. crystallinum, N. tabacum, O. sativa, P. kurroa, P. vulgaris, algae N. oloabundans, Z.mays
AS13 2746 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana