Anti-Enolase 2

Name: Anti-Enolase 2
SKU: AS10 651
Price: 319 EUR
Availability: InStock

Product no: AS10 651

AS10 651 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Arabidopsis thaliana, Helianthus annuus

319 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Recombinant Arabidopsis thaliana enolase UniProt: P25696-1, TAIR: At2g36530
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	200 �l
Reconstitution:	For reconstitution add 200 �l of sterile water
Storage:	Store lyophilized/reconstituted at -20�C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 2000 (WB)
Expected \| apparent MW:	47.7 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Helianthus annuus
Predicted reactivity:	Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

Application example

Western blot using anti-enolase antibodies

Coomassie staining of three recombinant sunflower ENO proteins after purification on IMAC column and SDS PAGE separation (A) Immunodetection carried out with the anti-Enolase antibody (B) (AS10 651 at 1:2000 dilution). The detection was done with the Goat Anti-Rabbit IgG (H+L) Alkaline phosphatase conjugated (AS09 607 at 1:5000 dilution). In (A) and (B), the lanes were loaded as follows: M indicates the molecular weight markers Lane 1- Recombinant (6xHis)HaENO2 (cytosolic and active isoform) Lane 2- Recombinant (6xHis)ΔHaENO1 (plastidial isoform with the N-terminal transit peptide removed) Lane 3- Recombinant (6xHis)HaENO3 (cytosolic and inactive isoform) In panel (A), 0.7 µg protein was loaded per lane. In panel (B) 50 ng protein was loaded per lane. The faint band seen below the main band in lane 1 in (B) is likely a degradation product of the recombinant protein. No band was detected in lanes 2 and 3.

Recombinant sunflower enolases are described Troncoso-Ponce et al. Plant Science (2018) 272:117-130).

Courtesy of Dr. Jean Rivoal, IRBV, Université de Montréal, Canada

Additional Information
Additional information (application): Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below
Background

Background:

Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate.

Alternative name: Bifunctional enolase 2/transcriptional activator

Product Citations

Selected references:

Zhang et al. (2020). A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11(1):4509.doi: 10.1038/s41467-020-18234-w.
Zhang et al. (2018). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.
Chen et al.(2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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