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Anti-Wheat gliadin
Product no: AS19 4331
AS19 4331 | Clonality: Polyclonal | Host: Rabbit | Reactivity: wheat gliadin
241
€
Delivery:
7-10 business days
- Product Info
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Immunogen: Wheat Gliadin (Native Protein) Host: Rabbit Clonality: Polyclonal Purity: >95%, Protein G purified to a total immunoglobulin G fraction. Format: Liquid Quantity: 50 µg Storage: Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western Blot (WB) Recommended dilution: 1 : 1000-1: 5000 (WB) Expected | apparent MW: 35 | 35 kDa - Reactivity
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Confirmed reactivity: wheat gliadin Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
2 and 10 µg of wheat flour were loaded/well and separated on 10 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at 2.µg/ml for 1h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions. - Additional Information
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Additional information: Product contains a preservative: 0,03% Proclin 300 and 50% Glycerol, 10 mM PBS, pH 7,4 - Background
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Background: Wheat gliadin is a class of proteins being a main component of gluten fraction and present in wheat and other cereals. - Protocols
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ELISA protocol
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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