Anti-VSV-G tag | Vesicular stomatitis virus (clone P5D4)
- Product Info
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Sub class: IgG1, κ Immunogen: VSV-G tagged protein, sequence: N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C Host: Mouse Clonality: Monoclonal Purity: Purified on Protein A Format: Liquid in PBS Quantity: 100 µg Storage: Store at -20°C; Avoid repeated freeze/thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. For long term storage, transfer to -80°C Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 0.125 -10 µg (ELISA) Expected | apparent MW: Depends upon fusion partner - Reactivity
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Confirmed reactivity: VSV-G tag - Application Examples
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ELISA with anti-VSV-G antibodies diluted in PBS containing 1% BSA was tested on a biotinylated VSV-tagged single chain antibody captured on immobilized Neutravidin. Biotinylated BSA was included as negative control. - Background
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Background: VSV-G tag corresponds to the partial peptide sequence of the vesicular stomatitis virus glycoprotein from Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G).. This epitope tag can be added to a protein from any species to allow further protein detection using different techniques like: immunolocalization, immunoprecipitation or Western blot. - Protocols
- Antibody protocols
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