Anti-V5 (clone V5,E10)
Product no: AS15 3027
AS15 3027 | Clonality: monoclonal | Host: Mouse | Reactivity: V5-tagged fusion proteins
274
€
Delivery:
3-6 business days
- Product Info
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Sub class: IgG1 Immunogen: KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide
Host: Mouse Clonality: Monoclonal Purity: Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide. Format: Liquid Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB) - Reactivity
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Confirmed reactivity: V5-tagged fusion proteins Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally Additional information (application): Antibody is purified on Protein A.
For western blot incubate antibody for one hour at room temperature. - Background
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Background: V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection. - Reviews:
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