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Anti-UBC7/13/14 | Ubiquitin-conjugating enzyme E2 7/13/14
AS23 4886 | Clonality: Polyclonal| Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana UBC7 UniProt: Q42540, TAIR: AT5G59300, UBC13: UniProt: Q42541, TAIR: AT3G46460 UBC14: UniProt: F4IWU7 TAIR: AT3G55380 Host: Rabbit Clonality: Polyclonal Purity: Antigern affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 - 1: 5000 (WB) Expected | apparent MW: 18.8 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana
Predicted reactivity: Brassica napus, Nicotiana tabacum, Solanum lycopresicum, Solanum tuberosum, Pisum sativum
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
1 - 30 µg total proteins of Arabidopsis thaliana 10-day-old seedlings; wild type
2 - 30 µg total proteins of Arabidopsis thaliana 10-day-old seedlings; ubc7-1
3 - 30 µg total proteins of Arabidopsis thaliana 10-day-old seedlings; ubc13-1
4 - 30 µg total proteins of Arabidopsis thaliana 10-day-old seedlings; ubc14-1
5 - 30 µg total proteins of Arabidopsis thaliana 10-day-old seedlings; ubc7/13/14
MW markers: BioRad Precision Plus Protein standards (bands indicated at the left)30 µg/lane) of total proteins extracted freshly from 10-day-old seedlings of Arabidopsis thaliana wt and mutants, with the exaction buffer (50 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM DTT, 1% (v/v) Triton X-100, Sigma protease inhibitor cocktail) and denatured with 4X SDS sample buffer at 95°C for 5 min. Samples were separated using 10% SDS-PAGE and transferred onto PVDF membrane (0.2 µm pore size), using wet transfer. After blocking with 5% milk in PBS-T for 0.5h at RT (room temperature) with agitation, the blot was incubated in the primary antibody at a dilution of 1: 5000 (from the initial antibody solution at 1 µg IgG/µl) in PBS-T at 4°C with agitation. The antibody solution was decanted, and the blot was washed 4 times, each for 10 min, in PBS-T at RT with agitation. The blot was incubated in the secondary antibody goat anti-rabbit IgG horse radish peroxidase conjugated, 1:10 000 for 1h at RT with agitation. It was washed and developed with Agrisera ECL SuperBright.
University of Saskatchewan, Canada
Note: Background signal can be decreased with further protocol adjustment.
Courtesy of Dr. Hong Wang, - Background
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Background: UBC proteins accept the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins and is involved in the formation of multiubiquitin chains as well as signals the proteins for selective degradation. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)