DISCONTINUED Anti-PTOX | Plastid terminal oxidase

Product no: AS16 3692
AS16 3692  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Araidopsis thaliana, Chlamydomonas reinhardtii

Replaced by AS23 4895
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  • Product Info
  • Immunogen:
    Part of Arabidopsis thaliana recombinant plastid terminal oxidase, TAIR: At4g22260, UniProt: Q56X52
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 µl
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 4000 (WB)
    Expected | apparent MW:

    30 | 37-41 kDa (Arabidopsis thaliana)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum, Spinacia oleracea
    Predicted reactivity: Higher plants
    Species of your interest not listed? Contact us
    Not reactive in: Galdieria sulphuraria, Phaeodactylum tricornutum
  • Application Examples
  • Application example

    Western blot using anti-PTOX antibodies     15 µg from total protein leaf extract of Spinacia oleracea (1), Arabidopsis thliana (2), Solanum lycopersicum (3), Nicotiana tabacum (4), Hordeum vulgare (5), Arabidopsis thaliana thylakoid fraction (6) were separated on SDS-PAGE and blotted to PVDF membrane. Incubation with primary antibody: 1: 4000 followed by chemiluminescent detection. Exposure time 2 minutes.

    Cross reactivity to 52 kDa band can be eliminated by using thylakoid fraction or blocking with 10 % milk ON/4°C.
  • Additional Information
  • Additional information: This product can be sold containing proclin if requested
    Additional information (application): In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended
  • Background
  • Background:
    PTOX (plastid terminal oxidase) is a component of electron transfer chain responsible for desaturation of phytoene, which prevents the generation of reactive oxygen species. It is involved in the differentiation of plastids: chloroplasts, amyloplasts, and etioplasts. PTOX is expressed ubiquitously in plant tissues and is located in the lumen.
    Synonymes: IM, IM1, immutants, AOX4, alternative oxidase 4, ubiquinol oxidase 4, chloroplastic/chromoplastic
  • Product Citations
  • Selected references: Urban, Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105024
    Pralon et al. (2020). Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in Arabidopsis thaliana. Front. Plant Sci., 25 March 2020
    Bolte et al. (2020). Dynamics of the localization of the plastid terminal oxidase PTOX inside the chloroplast. J Exp Bot. 2020 Feb 15. pii: eraa074. doi: 10.1093/jxb/eraa074.
    Cournac et al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54
    Cournac et al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62.
    Josse et al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
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    Number of reviews: (2)
    Monika | 2019-09-24
    0 | 0
    using chloroplasts isolated from A. thaliana dilution 1:4000 of antibody applied overnight at 4 degrees is O.K.
    Ulusan | 2017-05-08
    0 | 0
    is very good

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