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Heat shock

Heat shock

Important note: Please note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experiences temperatures that are 10 degrees higher than the growing temperature. Therefore, the HSPs induction temperatures for plants grown at 18°C differ from these for plants grown at 24°C. Another very effective parameter is the humidity. When using low humidity, the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.

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Confirmed reactivity

Agave tequilana Agave tequilana var. Weber Agrostis stolonifera cv. Penncross Algae Arabidopsis thaliana Brachypodium distachyon Brassica juncea Brassica napus Brassica oleracea Chlamydomonas reinhardtii Chlamydomonas sp. UWO241 Citrus sp. Cucumis sativus Cucumis sativus var. Krak Cyanobacteria Desmodesmus subspicatus Diatoms Dinoflagellates Eragrostis tef Fagopyrum esculentum Glycine max Gracilaria vermiculophylla Higher plants Hordeum spontaneum Hordeum vulgare Hsp70 Iris pumila Lichens Lupinus angustifolius Medicago sativa Mosses Nicotiana tabacum Oryza sativa Physcomitrium patens Picrorhiza kurroa Pinellia ternata Pinus strobus Pinus sylvestris Pisum sativum Plasmodium falciparum Polyscias elegans Recombinant sHSP with ACD domain Salicornia sp. Setaria italica Silene vulgaris Solanum lycopersicum Solanum tuberosum Sorghum bicolor Synechococcus sp. PCC 7942 Synechocystis sp. 6803 GT Synechocystis sp. PCC 6803 Thellungiella salsuginea Trebouxia TR1 Trebouxia TR9 Triticum aestivum Ulva prolifera Vicia faba Zea mays

Predicted reactivity

ACD domain of sHSP Aegilops kotschyi Ageratina adenophora Allium sativum Anabaena sp. Arabidopsis thaliana Arabis alpina Arachis diogoi Arundo donax Beta vulgaris Brachypodium distachyon Brassica napus Brassica rapa subsp. pekinensis Camellia sinensis Capsella rubella Chlamydomonas reinhardtii Chlorella vulgaris Citrus clementina Citrus sinensis Coffea arabica Coffea canephora Crocosphaera watsonii Cucumis sativus Cyanobacteria Cyanothece sp. PCC 7424 Dicots Dunaliella salina Eriobotrya japonica Francisella sp. Fraxinus sp. Glycine max Glycine soja Gossypium arboreum Gossypium hirsutum Helianthus annuus higher plants Hordeum vulgare Hordeum vulgare var. distichum Linum usitatissimum Lotus japonicus Lyngbya sp. PCC 8106 Medicago sativa Medicago truncatula Microcoleus chthonoplastes PCC 7420 Micromonas pusilla Monocots Musa acuminata Musa acuminata subsp. Malaccensis Nannochloropsis gaditana Nicotiana benthamiana Nicotiana tabacum Nodularia spumigena CCY 9414 Nostoc sp. Olea europaea Oryza sativa Ostreococcus lucimarinus Ostreococcus sp. Phaseolus vulgaris Phtheirospermum japonicum Physcomitrium patens Picea sitchensis Pinus taeda Pisum sativum Populus balsamifera Populus trichocarpa Prunus persica Ricinus communis Rosa chinensis Saccharum sp. Salix gilgiana Saussurea medusa Solanum commersonii Solanum lycopersicum Solanum tuberosum Sorghum bicolor Spinacia oleracea Synechococcus sp. Synechococcus sp. PCC 7942 Theobroma cacao Thermosynechococcus sp. Tragopogon dubius Tragopogon porrifolius Triticum aestivum Triticum sp. Vitis vinifera Volvox sp. Zea mays

Tested applications

ELISA (ELISA) Immunoprecipitation (IP) Protein extraction Western blot (WB)

Clonality

Monoclonal Polyclonal

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1 | PEB (4x) | protein extraction buffer

AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

123 €

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