Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody)

The expression and stability of PIF4 and HY5 are spatially regulated.a, b RT-qPCR analysis of HY5 and PIF4. RT-qPCR samples were from Col-0 whole seedlings, shoot or root grown for 5 days at 22?°C and then either kept at 22?°C or transferred to 28?°C for 4?h. Three biological repeats were performed. Relative gene expression levels were normalized using the expression levels of ACT7 as well as the expression levels of shoot. Asterisks indicate statistically significant difference using two-sided Student’s t-test; **p?<?0.01. P values for HY5 root 22 vs 28: 0.0009, PIF4 whole seedlings: 0.0005, PIF4 shoot: 0.0004, respectively. Error bars indicate SD (n?=?3). c Western blots show the level of native PIF4 and HY5 in the shoot and root samples separated from whole seedlings grown for 5 days at 22?°C and then either kept at 22?°C or transferred to 28?°C for 4?h. hy5-215 and pifQ were used as negative controls. * and ** indicate root and shoot specific cross-reacting bands. *** indicate cross-reacting band in HY5 blot. Red numbers indicate the relative values for PIF4 or HY5 band divided by the cross-reacting band. Two independent experiments were repeated with similar results. d Simplified scheme showing spatial regulation of thermomorphogenesis by distinct transcription factor-kinase module. PIF4 and HY5 are main hub transcription factors in the shoot and root thermomorphogenesis, respectively, while PIF4 and HY5 both contribute to hypocotyl thickness. The abundance and/or activity of PIF4 and HY5 are regulated by SPA-mediated phosphorylation. As a thermosensor, phyB directly inhibits PIF4 and indirectly stabilizes HY5 to coordinately regulate shoot and root thermomorphogenesis.