Anti-Nitrotyrosine (mouse monoclonal) (clone EM-30)

Product no: AS21 4622
AS21 4622 | Clonality: Monoclonal | Host: Mouse | Reactivity: nitrotyrosine
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  • Product Info
  • Sub class: IgG1
    Immunogen: NO2-Tyr-CH2-Thyroglobulin
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin purified on Protein A.
    Format: Liquid at 1 mg/ml.
    Quantity: 0.1 mg
    Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunohistochemistry (IHC) paraffin, Iimmunohistochemistry (IHC) frozen sections, Western blot (WB)
    Recommended dilution: (IHC) paraffin: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
    (IHC) frozen sections: 10 µg/ml; positive tissue: colon cancer epithelium, prostate hyperplasia.
    (WB): positive control: NO2-BSA, NO2-HSA.
  • Reactivity
  • Confirmed reactivity: Nitrotyrosine
  • Application Examples
  • IHC using mouse monoclonal anti-nitrotyrosine antibodies

    Human tumoral colon epithelium stained (frozen sections) with anti-nitrotyrosine antibodies (clone EM-30).

  • Additional Information
  • Additional information: This antibody detects nitrotyrosine group in biological material, It can be used as a marker for peroxynitrite formation in particular tissues
  • Background
  • Background: Nitrotyrosine can be detected in proteins from a variety of tissues, usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Antibody Protocols
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