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Anti-JAZ1 | Jasmonate ZIM-domain protein 1
AS13 2648 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana JAZ1 protein sequence, UniProt: Q9LMA8, TAIR: AT1G19180
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000-1 : 3000 (WB) Expected | apparent MW: 27.6 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Not reactive in: Gossypium hirsutum, Nicotiana tabacum, Oryza sativa, Solanum tuberosum
- Application Examples
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Application example
JAZ1HA was transiently expressed in Arabidopsis thaliana protoplast for 6 hours. 200 µl of protoplast cells were harvested and mixed with 100 µl 2X SDS buffer. Proteins were separated on 4-12% NuPAGE gel and blotted for 1 hr to PVDF using the Invitrogen XCell II blot module. Blot was blocked with 5% non-fat milk in TBST buffer for 1 hr at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 over night at 4°C with agitation in TBST with 5% non-fat milk. The blot was rinsed briefly twice, then washed 3 times with TBST for a total of 45 min at RT. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1: 15 000 for 1 hr at RT in TBST with 5% non-fat milk. The blot was washed as above and developed for 5 min using the SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and exposed for 2 min.
Courtesy of Dr. Xuecheng Zhang, Department of Molecular Biology Massachusetts General Hospital, USA - Background
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Background: JAZ1 (Jasmonate-ZIM domain containing protein 1) acts as a repressor of jasmonate responses which are negatively regultated by the proteasome. Interacts with COI1 and inositol pentakisphosphate.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
- Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS12 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
Choose the appropriate volume in the drop down menu to the right