Anti-HSP90-1 | heat shock protein 90-1

Name: Anti-HSP90-1 | heat shock protein 90-1
SKU: AS08 346
Price: 319 EUR
Availability: InStock
Rating: 5 (1 reviews)

Product no: AS08 346

AS08 346 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. distachyon, Chlamydomonas sp. UWO241, H. vulgaris, Salicornia sp., S. lycopersicum, Z. mays

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DATA SHEET IN PDF

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Product Info

Immunogen:	Full-length recombinant Hsp90-1 from Arabidopsis thaliana, UniProt: P27323-1, TAIR: AT5G52640. Cytoplasmic isoform.
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	80.6 \| 95 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Brachypodium distachyon, Brassica napus, Chlamydomonas sp. UWO241, Fagopyrum esculentum, Hordeum vulgare, Salicornia sp., Solanum lycopersicum, Zea mays, Vicia faba
Predicted reactivity:	Fraxinus sp., Glycne max, Linum usitatisimum, Micromonas pulsilla, Musa acuminata, Nicotiana benthamina, Nicotiana tabacum, Oryza sativa, Ostreococcus lucimarinus, Physcomitrium patens, Populus balsamifera, Ricinus communis, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

Application examples:

Reactant: Solanum tuberosum (Potato)

Application: Western Blotting

Pudmed ID: 26612975

Journal: Mol Breed

Figure Number: 3B

Published Date: 2015-11-28

First Author: So?tys-Kalina, D., Szajko, K., et al.

Impact Factor: 2.533

Open Publication

a Expression level of the Hsp90 gene in tubers after cold storage (CS); ns non-significant differences between parental clones (Student’s t test); a and b homogenous groups between bulk samples LII and DII (Tukey’s test). b Amounts of Hsp90-1 and Hsp90-2 protein isoforms in tubers after cold storage (CS); Marker—Prosieve® QuadColor™ Protein Marker. Parental clones: DG 97-952 (chip color 7) and DG 08-26/39 (chip color 4); bulk samples: LII (chip color 8–9) and DII (chip color 1–3)

Additional Information

Additional information:

Antibody is recognizing both, heat inducible Hsp90-1 and constitutive isofrom Hsp90-2. Both proteins have ca. 85% similarity.

This product can be sold containing ProClin if requested

Background

Background:

HSP90-1 (heast shock protein 90-1) is an isoform involved in response to bacterium, arsenic and heat. Synonymes: ATHS83; ATHSP90.1; F6N7.13; F6N7_13; HEAT SHOCK PROTEIN 81-1; HEAT SHOCK PROTEIN 83; HEAT SHOCK PROTEIN 90.1; HSP81-1; HSP81.1; HSP83.

Product Citations

Selected references:

Vuković et al. (2024). Heat Priming Modifies Heat Stress Response in BPM1-Overexpressing Arabidopsis thaliana (L.) Heynh. J Olant Growth Regulation. https://doi.org/10.1007/s00344-024-11337-4.
Llamas et al. (2023). In planta expression of human polyQ-expanded huntingtin fragment reveals mechanisms to prevent disease-related protein aggregation. Nat Aging. 2023 Nov;3(11):1345-1357.doi: 10.1038/s43587-023-00502-1.
Kis et al. (2023). Targeted mutations in the GW2. 1 gene modulate grain traits and induce yield loss in barley. Plant Science Available online 27 December 2023, 111968.
Tokić, M.; Leljak Levanić, D.; Ludwig-Müller, J.; Bauer, N. Growth and Molecular Responses of Tomato to Prolonged and Short-Term Heat Exposure. Int. J. Mol. Sci. 2023, 24, 4456.
Zeng et al. (2023) HSP90s are required for hypocotyl elongation during skotomorphogenesis and thermomorphogenesis via the COP1-ELF3-PIF4 pathway in Arabidopsis [published online ahead of print, 2023 Jan 27]. New Phytol. 2023;10.1111/nph.18776. doi:10.1111/nph.18776
Jiang et al. (2022) CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice. Biotechnol Biofuels Bioprod. 2022;15(1):111. Published 2022 Oct 14. doi:10.1186/s13068-022-02205-y
Belonoznikova et al. (2022). Seed Protection of Solanum lycopersicum with Pythium oligandrum against Alternaria brassicicola and Verticillium albo-atrum. Microorganisms. 2022 Jul 4;10(7):1348. doi: 10.3390/microorganisms10071348. PMID: 35889067; PMCID: PMC9315653.
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.
Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.
Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203
Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.
Shteinberg et al. (2021) Tomato Yellow Leaf Curl Virus (TYLCV) Promotes Plant Tolerance to Drought. Cells. 2021 Oct 25;10(11):2875. doi: 10.3390/cells10112875. PMID: 34831098; PMCID: PMC8616339.
Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.
Ojeda et al. (2021) A chloroplast redox relay adapts plastid metabolism to light and affects cytosolic protein quality control, Plant Physiology, 2021;, kiab246.
Plazek et al. (2020). Synthesis of heat-shock proteins HSP-70 and HSP-90 in flowers of common buckwheat (Fagopyrum esculentum) under thermal stress. Crop and Pasture Science, 71(8), 760-767, July 2020
Esteve-Bruna et al. (2020). Prefoldins Contribute to Maintaining the Levels of the Spliceosome LSM2-8 Complex Through Hsp90 in Arabidopsis. Nucleic Acids Res. 2020 May 12;gkaa354. doi: 10.1093/nar/gkaa354.
Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.
Gorovits et al. (2020). Pharmaceuticals in treated wastewater induce a stress response in tomato plants. Sci Rep. 2020 Feb 5;10(1):1856. doi: 10.1038/s41598-020-58776-z.
Sedaghatmehr et al. (2019). A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. 2019 Mar;42(3):1054-1064. doi: 10.1111/pce.13426.
Kato et al. (2019). Induction of the heat shock response in Arabidopsis by chlorinated 1,4-naphthoquinones. Plant Growth Regul (2019).
Balazova et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.
Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018.
Sedaghatmehr et al. (2018). A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. 2018 Aug 22. doi: 10.1111/pce.13426.
Danilova et al. (2018). Differential impact of heat stress on the expression of chloroplast-encoded genes. Plant Physiol Biochem. 2018 May 23;129:90-100. doi: 10.1016/j.plaphy.2018.05.023.
Gil et al. (2017) ZEITLUPE Contributes to a Thermoresponsive Protein Quality Control System in Arabidopsis. PlantCell. 2017 Nov;29(11):2882-2894. doi: 10.1105/tpc.17.00612.
Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715
Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.
Moshe et al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275.
Svozil et al. (2015). Proteasome targeting of proteins in Arabidopsis leaf mesophyll, epidermal and vascular tissues. Front Plant Sci. 2015 May 28;6:376. doi: 10.3389/fpls.2015.00376. eCollection 2015.
Tillmann et al. (2014). Hsp90 is involved in the regulation of cytosolic precursor protein abundance in tomato. Mol Plant. 2014 Oct 20. pii: ssu113.
Svozil et al. (2014). Protein abundance changes and ubiquitylation targets identified after inhibition of the proteasome with Syringolin A. Mol Cell Proteomics. 2014 Apr 13.
Finka et al. (2012). Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance. Plant Cell, June 2012.
Stachurska, J.; Sadura, I.; Rys, M.; Dziurka, M.; Janeczko, A. Insight into Hormonal Homeostasis and the Accumulation of Selected Heat Shock Proteins in Cold Acclimated and Deacclimated Winter Oilseed Rape (Brassica napus L.). Agriculture 2023, 13, 641.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

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Number of reviews: (1)

Ernesto Llamas | 2023-06-21

0 | 0

I used this antibody for detecting Hsp90-1 in Arabidopsis thaliana tissues and works very well. I load a total of 25ug of protein using a dilution 1:3000 of the antibody for developing western blots.

Ernesto Llamas- University of Cologne