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Anti-HSP90C | alfa-HSP90C, heat shock protein (algal)
AS06 174 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
- Product Info
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Immunogen: C-terminal 238 amino acids of HSP90C, Q66T67, expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1 : 3000 (WB) Expected | apparent MW: 89 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Ostreococcus sp.
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): HSP90C protein is easily degraded and degradation products are detected by this antibody - Background
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Background: HSP90C is a nuclear-encoded, chloroplast-targeted chaperone of the HSP90 family. It forms dimers that interact with HSP70B. HSP90C displays a weak ATPase activity that is inhibited by radicicol.
- Product Citations
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Selected references: Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203
Perlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.
Willmund & Schroda (2005). HSP90C is a bona-fide Hsp90 that interacts with plastidic HSP70B in Chlamydomonas reinhardtii. Plant Phys. 138, 2310–2322.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
AS08 346 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. distachyon, Chlamydomonas sp. UWO241, H. vulgaris, Salicornia sp., S. lycopersicum, Z. mays
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)