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Anti-HDT3 | Histone deacetylase HDT3
AS16 3968 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana HDT3, UniProt: Q9LZR5, TAIR: At5g03740
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 4000-1 : 8000 (WB) Expected | apparent MW: 31.8 | 40 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Noccaea caerulescens
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example
Nuclei were isolated from Arabidopsis thaliana leaves and resuspended with SDS loading buffer and then denatured at 95oC for 10 min. Proteins were separated on 12% SDS-PAGE and blotted 1h to nitrocellulose membrane using semi-dry or tank transfer. Blots were blocked with 3% milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at 1:4 000 dilution for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBST at RT with agitation. Blot was incubated in radish peroxidase conjugated goat anti-rabbit IgG (AS09 602, Agrisera) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 1 min with chemiluminescent detection reagent. Exposure time was 100 seconds.
Note: increasing blocking to 10% milk may reduce background signal.
Courtesy of Dr. Xiangsong Cheng, Wisconsin Institute for Discovery, USA - Background
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Background: HDT3 is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. This protein is also involved in the modulation of abscisic acid and stress-responsive genes.Synonymes:HD-tuins protein 3, Histone deacetylase 2c
- Product Citations
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Selected references: Park et. al (2018). Epigenetic switch from repressive to permissive chromatin in response to cold stress. Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):E5400-E5409. doi: 10.1073/pnas.1721241115. - Protocols
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS10 710 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. cinerea, C. reinhardtii, H. vulgare, S. lycopersicum, V. faba, P. tricornutum, P. patens, S. europaea, Z. mays | cellular [compartment marker] of nucleoplasm
AS11 1792 | Clonality: Polyclonal | Host: Rabbit | predicted Reactivity: Arabidopsis thaliana