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Anti-H+ATPase | Plasma membrane H+ATPase (rabbit antibody), HRP-conjugated (40 ”g)
AS07 260-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane
Benefits of using this antibody
- Product Info
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Immunogen: KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP. Format: Liquid Quantity: 40 µg Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: 1 : 600-1 : 1000 (IF), 1 : 1000-1 : 5 000 (WB) Expected | apparent MW: 90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
- Reactivity
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Confirmed reactivity: Actinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Zea mays, Vicia faba Predicted reactivity: Algae, Amaranthus hypochondriacus,Avena sativa,Beta vulgaris, Cyanidioschyzon merolae, Dunaliella spp., Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Prosopis alba, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis
Species of your interest not listed? Contact usNot reactive in: Allium sp., Aspergillus niger, Citrus limon, Colobanthus apetala, Cuminium cyminum, Curcuma amada, Deschampsia antractica, Lupinus luteus, Morinda citrifolia, Trigonella foenum, Vicia faba
- Additional Information
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Additional information: Cellular [compartment marker] for plasma membrane
Additional information (application): VERY IMPORTANT: Please, do not heat up your samples above 70°C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot.
Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates.
H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents. - Background
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Background: The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
This antibody is directly conjugated to HRP. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Plasma membrane protein isolation protocol - Reviews:
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Accessories
AS07 213 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants including A.comosus, A.thaliana, C.sativus, C. australis R.Br, C.reinhardtii, F. margarita Swingle, H.vulgare, L.esculentum, L.longiflorum, Malus x domestica Borkh. c.v. Fuji, M. truncatula, M.crystallinum, N.tabacum, N.caerulescens, O.sativa, P.hybrida cv. Mitchell, Populus sp., P.vittata, Thellungiella sp., T. aestivum, Z.mays, V. vinifera | Cellular [compartment marker] of tonoplast membrane
Trial size limited to one per customer
AS11 1754 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C.reinhardtii, L. angustifolius, P. sativum, S. tuberosum, Synechococcus sp. PCC 7942, Z. mays L.
AS05 085-10 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases
Benefits of using this antibody