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Anti-H3K27me3 | Histone H3, trimethylated lysine 27 (H3K27me3)
Product no: AS16 3193
AS16 3193 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C. elegans, Human, Mouse, Populus sp. , S. lycopersicum, Z. mays
503
€
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated synthetic peptide Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum. Format: Liquid Quantity: 50 µg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Chromatin Immunoprecipitation Sequencing (ChIP-seq), Dot blot (Dot), ELISA (ELISA), Immunofluorescence (IF), peptide array (PA), Western blot (WB) Recommended dilution: 0.5-1 µg/IP (ChIP-seq), 1 : 20 000 (Dot), 1 : 1000 (ELISA), 1 : 500 (IF), 1 : 20 000 (PA), 1 : 1000 (WB) Expected | apparent MW: 15 kDa
- Reactivity
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Confirmed reactivity: Human Predicted reactivity: Arabidopsis thaliana, Caenorhabditis elegans, Mouse, Populus sp., Solanum lycopersicum, Zea mays Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example 
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using anti-H3K27me3 antibodies. The antibody was diluted 1:1 000 in TBS-Tween containing 5% skimmed milk. - Additional Information
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Additional information: Antibody is provided in PBS containing 0.05% azide and 0.05% ProClin 300 - Background
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Background: Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Methylation of histone H3K27 is associated with inactive genomic regions. - Reviews:
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