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Anti-GST tag (mouse monoclonal) (clone S-tag-05)
- Product Info
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Sub class: IgG2b Immunogen: GST protein Host: Mouse Clonality: Monoclonal Purity: Immunoglobulin purified on Protein A. Format: Liquid at 1 mg/ml. Quantity: 0.1 mg Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1 µg/ml (WB) - Reactivity
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Confirmed reactivity: GST fusion proteins - Application Examples
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Samples:
Lane 1: affinity purification of Glutathione-S-Transferase (GST) overexpressed in E. coli, using commercial GST sorbent
Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared with anti-GST monoclonal antibodies - Additional Information
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Additional information: This antibody is recognizing native and denatured fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. It can be also used for immuno purification of GST-tagged proteins. - Background
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Background: GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags. - Protocols
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Agrisera Western Blot protocol and video tutorials
Antibody Protocols - Reviews:
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Accessories
AS09 627 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
This product can be purchased in 3 different volumes:
AS16 ECL-N-10, 10 ml. Trial size limited to one per customer
AS16 ECL-N-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
AS17 4147 | Clonality: Polyclonal | Host: Rabbit | Reactivity: GST-tagged proteins