Goat anti-Human IgG (H&L), HRP conjugated
Product no: AS10 764
AS10 764 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (heavy and light chains, H&L)
175
€
Delivery:
3-6 business days
- Product Info
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Immunogen: Purified human IgG, whole molecule
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Heavy chains on human IgG and light chanins on all human immunoglobulins Not reactive in: Non immunoglobulin human serum proteins - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of ProClin 150 is used as preservative - Background
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Background: Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
- Protocols
- Antibody protocols
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