Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated
AS10 1039 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG + IgA + IgM
- Product Info
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Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified IgG, F(ab)'2 fragment. Format: Lyophilized Quantity: 0.5 mg Reconstitution: For reconstitution add 0.55 ml of sterile water Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Recommended dilution: 1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB) - Additional Information
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Additional information: This antibody reacts with the heavy chains on human IgG + IgA + IgM, and the light chains on all human immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, Protease/IgG free and 0.1% (v/v) Kathon CG is used as preservative.
- Background
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Background: Goat anti-human IgG + IgA + IgM, HRP conjugated is a F(ab)'2 fragment, of a secondary antibody which binds to human IgG+IgA+IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG. - Product Citations
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Selected references: Wielkoszynski et al. (2018). Novel diagnostic ELISA test for discrimination between infections with Yersinia enterocolitica and Yersinia pseudotuberculosis. Eur J Clin Microbiol Infect Dis. 2018 Dec;37(12):2301-2306. doi: 10.1007/s10096-018-3373-9. - Protocols
- Antibody protocols
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Accessories
- 10ml trial size is restricted to one unit per customer -
AS15 HRPstab | Bovine and mammalian protein free HRP and ALP-conjugate and protein stabilizer
This product can be purchased in 2 different volumes:
AS15 HRPstab-10, 10 ml. Trial size limited to one per customer
AS15 HRPstab-100, 100 ml
Choose the appropriate volume in the drop down menu to the right