Goat anti-Human IgG (H&L), F(ab)'2 fragment, HRP conjugated, min, cross-reactivity bovine/mouse/rabbit serum
AS10 785 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (H&L), F(ab)'2 fragment
- Product Info
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Immunogen: Purified human IgG (H&L)
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Liquid Quantity: 0.5 mg Storage: Store non-diluted antibody at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: The optimal working dilution should be determined by the investigator - Reactivity
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Confirmed reactivity: Human IgG (H&L), F(ab)'2 fragment Predicted reactivity: Human IgG (H&L), F(ab)'2 fragment Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10% (w/v) BSA, Protease/IgG free
0.1% (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG
Additional information (application): This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.
No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
- Background
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Background: Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
- Protocols
- Antibody protocols
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