Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated

Product no: AS10 774

AS10 774 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG (H&L)

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  • Product Info
  • Immunogen:

    Purified human IgG

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
    Format: Liquid
    Quantity: 0.5 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50% to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
  • Reactivity
  • Confirmed reactivity: Human IgG (H&L)
    Predicted reactivity: Human IgG (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

    Purity is ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.

    Additional information (application):

    This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis.

    No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

  • Background
  • Background:

    Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

  • Protocols
  • Antibody protocols
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