Anti-GFP | Green fluorescent protein (VENUS) - DyLight® 650 conjugated (40 µg)

Name: Anti-GFP | Green fluorescent protein (VENUS) - DyLight® 650 conjugated (40 µg)
SKU: AS18 4227-DL650
Price: 490 EUR
Availability: InStock

Product no: AS18 4227-DL650

AS18 4227-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Venus

490 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Full length, recombinant VENUS protein, expressed in E.coli, UniProt: P42212
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.
Format:	Liquid in PBS pH 7,4.
Quantity:	40 µg
Storage:	Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
Tested applications:	Immunofluorescence (IF), Western blot (WB)
Recommended dilution:	To be determined by end user.
Expected \| apparent MW:	Depends upon fusion partner

Reactivity
Confirmed reactivity: VENUS

Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
Additional Information
Additional information: DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
Background

Background:

Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr), It is an energy-transfer acceptor, Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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