Anti-FREE1 | Protein FREE1

Name: Anti-FREE1 | Protein FREE1
SKU: AS22 4702
Price: 319 EUR
Availability: InStock

Product no: AS22 4702

AS22 4702 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana

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DATA SHEET IN PDF

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Product Info

Immunogen:	KLH-conjugated peptide derived from Arabidopsis thaliana FREE1 protein sequence, UniProt: Q9ASS2, TAIR: At1g20110
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Antigen affinity purified serum, in PBS pH 7.4
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution, add 50 µl, of sterile or deionized water.
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 1000 - 1: 5000 (WB)
Expected \| apparent MW:	65.39 \| 75 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Application Examples

Western blot using anti-FREE1 antibodies

20 µg/well of total protein extracted from Arabidopsis thaliana seedlings. Exact buffer components were: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% TritonX-100, 1x protease inhibitor cocktail. Proteins were denatured with exact buffer components at 96°C/ 10 min. Samples were separated in the cold on 10 % SDS-PAGE and blotted for 1.5 h to nitrocellulose (pore size of 0.22 µm), using: wet transfer in the cold. Blot was blocked with 1 % milk for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h/RT with agitation in PBS-T with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in PBS-T for 45 min with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AgriseraBright (AS16 ECL-N). Exposure time was 120 s.

Courtesy of Dr. Caiji Gao, School of Life Sciences, South China University, Shipai, China

Additional Information
Additional information (application): FREE1 antibody is also recognizing recombinant FREE1 expressed under S35 promotor in Arabidopsis thaliana.
Background

Background:

FREE1 (Protein FREE1) is involved in the regulation of mulitivesicular/prevacuolar compartment protein sorting. Regulates multivesicular body (MVB) protein sorting and plant growth. Alternative names: FYVE domain protein required for endosomal sorting 1, FYVE domain-containing protein 1,FYVE1, PDE330, Pigment Defective Embryo 330.

Product Citations

Selected references:

Tanigawa et al. (2024). FYVE1/FREE1 is involved in glutamine-responsive TORC1 activation in plants. iScience. 2024 Aug 26;27(9):110814. doi: 10.1016/j.isci.2024.110814.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Poster Collection
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