Anti-SnRK2,2, SnRK2,3, SnRK2,6 | Ser/Thr-protein kinase SnRK

Name: Anti-SnRK2,2, SnRK2,3, SnRK2,6 | Ser/Thr-protein kinase SnRK
SKU: AS14 2783
Price: 319 EUR
Availability: InStock
Rating: 5 (1 reviews)

Product no: AS14 2783

AS14 2783 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Data sheet
Product Info

Immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2 UniProt:Q39192 ,TAIR: At3g50500, SnRK2.3, UniProt: Q39193 TAIR: At5g66880, SnRK2.6 UniProt: Q940H6, TAIR: At4g33950
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 1000 (WB)
Expected \| apparent MW:	41 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Brassica oleracea, Glycine max, Medicago sativa, Morsu notabilis, Oryza sativa, Phaseolus vulgaris, Populus sp., Triticum sp., Vicia faba Species of your interest not listed? Contact us
Not reactive in:	Chlamydomonas reinhardtii, Mesotaenium sp., Zygnema sp.

Application Examples

application example

western blot using anti SnrK 2.2/3/6 antibody

20 µg of total protein from two-week-old seedling of Arabidopsis thaliana Col-0 (1), snrk2.2/3/6 (2), snrk1/4/5/7/8/9/10 (3) extracted with kinase buffer were separated on 10 % SDS-PAGE and blotted 30 min to PVDF using semi-dry. Blots were blocked with 5% skim milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 in 0.5% TBST for overnight at 4 oC with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG goat peroxidase conjugated) diluted to 1: 5 000 in 0.5 % TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 30 seconds.

Dr. Pengcheng Wang, Purdue University, USA

Additional Information
Additional information: This product can be sold containing proclin if requested
Background

Background:

SnRK2.2, SnRK2.3, SnRK2.6 are members of SNF1-related protein kinases whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress. Involved in ABA signalling pathway.

Alternative names: OST1-kinase-like 2, Protein ATHPROKIN B, SNF1-related kinase 2.3, SnRK2.3, Protein OPEN STOMATA 1
SNF1-related kinase 2.6, SnRK2.6, Serine/threonine-protein kinase OST1, OST1-kinase-like 3, Protein ATHPROKIN A, SNF1-related kinase 2.2, SnRK2.2

Product Citations

Selected references:

Lu et al.(2024). N-glycosylation of SnRK2s affects NADPH maintenance in peroxisomes during prolonged ABA signalling. Nat Commun. 2024 Aug 5;15(1):6630. doi: 10.1038/s41467-024-50720-3.
Belda-Palazón et al. (2020) A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth. Nat Plants. 2020 Nov;6(11):1345-1353. doi: 10.1038/s41477-020-00778-w. Epub 2020 Oct 19. PMID: 33077877.
Wawer et al. (2018) mRNA Decapping and 5'-3' Decay Contribute to the Regulation of ABA Signaling in Arabidopsis thaliana. Front Plant Sci. 2018 Mar 12;9:312. doi: 10.3389/fpls.2018.00312.

Protocols
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Number of reviews: (1)

| 2016-07-26

0 | 0

The antibodies work very well for Arabidopsis plants, when using the protocol given by the manufacturer. Very strong single signal (around 41-42 kDa) was observed in leaves extracts and very weak for roots (25 ug of protein extract was used for both tissues).