Anti-eIF5 | Eukaryotic initiation factor eIF5

Product no: AS19 4252

AS19 4252  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Triticum aestivum

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319 €
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DATA SHEET IN PDF
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Recombinant eIF5 of Triticum aestivum
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 8000 (WB)
    Expected | apparent MW:

    49 | 50 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum
    Predicted reactivity: Nelumbo nucifera, Triticum urartu, Zea mays
    Species of your interest not listed? Contact us
  • Application Examples

  • Application example

    Western blot using anti-eIF5 antibodies

    Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.) Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 5 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF5 band. Courtesy Dr. Karen Browning, The University of Texas at Austin , USA
  • Background
  • Background:

    eIF5 (Eukaryotic initiation factor eIF5) is involved in protein biosynthesis. 
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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