Anti-eIF4A | Eukaryotic initiation factor eIF4A

Name: Anti-eIF4A | Eukaryotic initiation factor eIF4A
SKU: AS19 4251
Price: 319 EUR
Availability: InStock

Product no: AS19 4251

AS19 4251 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Lactuca sativa, Nicotiana tabacum,Triticum aestivum, Pisum sativum

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DATA SHEET IN PDF

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Product Info

Immunogen:	Recombinant eIF4A of Triticum aestivum
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1: 10 000 - 1 : 20 000 (WB)
Expected \| apparent MW:	47.2 \| 50 kDa

Reactivity

Confirmed reactivity:

Arabidopsis thaliana, Lactuca sativa, Nicotiana benthamiana, Nicotiana tabacum,Triticum aestivum, Pisum sativum

Predicted reactivity:

Aegilops tauschii, Actinidia chinensis var. chinensis, Albuca bracteata, Ananas comosus, Brachypodium distachyon, Carex duriuscula subsp. rigescens, Dichanthelium oligosanthes, Dorcoceras hygrometricum, Eleusine indica, Nicotiana benthamiana, Nicotiana sylvestris, Oryza sativa subsp. japonica, Panicum miliaceum, Pennisetum americanum, Phoenix dactylifera, Setaria italica, Sorghum bicolor, Trema orientale, Triticum aestivum, Zea mays

Species of your interest not listed? Contact us

Application Examples

Application example

Recommend incubation for 1 hr at room temperature in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST). Figure shows plant leaf extracts or wheat germ extract (5 microL of the supernatant of 50 mg liquid nitrogen powdered tissue/100 microL 2X SDS Laemmli buffer containing protease inhibitor crushed with a plastic pestle in a microfuge tube and centrifuged for 10 min at 4 C.). Image shown is using Agrisera Anti-rabbit HRP (1/30,000) for 1 hr at RT in 5% non-fat dry milk in phosphate-buffer saline with 0.5% Tween-80 (PBST) and using Agrisera Ultra Bright substrate and exposed for 2 seconds in a BioRad ChemDoc on high resolution. Blue star indicates eIF4A band.

Courtesy Dr. Karen Browning, The University of Texas at Austin , USA

Background
Background:
eIF4A (Eukaryotic initiation factor eIF4A) is involved in protein biosynthesis.
Product Citations

Selected references:

Li et al. (2021) Efficient and high-throughput pseudorecombinant-chimeric Cucumber mosaic virus-based VIGS in maize. Plant Physiol. 2021 Dec 4;187(4):2865-2876. doi: 10.1093/plphys/kiab443. PMID: 34606612; PMCID: PMC8644855.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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