Anti-DYKDDDDK (binds to Sigma FLAG®) (chicken antibody, polyclonal)
AS20 4379 | Clonality: Polyclonal | Host: Chicken | Reactivity: DYKDDDDK-tag
- Product Info
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Immunogen: KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).
Host: Chicken Clonality: Polyclonal Purity: Immunogen affinity purified in PBS pH 7.2. Contains 0,1 % sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunofluorsescence (IF), Western blot (WB) Recommended dilution: 1: 5000 - 1: 10 000 (ELISA), 1: 500 (IF), 1 : 2000 - 1: 10 000 (WB) - Reactivity
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Confirmed reactivity: DYKDDDDK epitope tag (Sigma FLAG®) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): Antibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40 - Background
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Background: Chicken anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®). - Protocols
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Agrisera Western Blot protocol and video tutorials
Antibody protocols - Reviews:
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Accessories
AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag
Replaced by AS20 4442
AS15 2871 | Clonality: monoclonal | Host: Mouse | Reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins