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Donkey anti-Sheep IgG (H&L), DyLight® 650 conjugated, min, cross-reactivity to human or rabbit IgG
AS12 2353 | Clonality: Polyclonal | Host: Sheep | Reactivity: Sheep IgG (H&L)
- Product Info
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Immunogen: Purified Sheep IgG, whole molecule
Host: Donkey Clonality: Polyclonal Purity: Immunogen affinity purified donkey IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Recommended dilution: 1 : 20-1 : 2000 for most applications - Additional Information
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Additional information: Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins
No reactivity is observed to: non-immunoglobulin sheep serum proteins.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. - Background
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Background: Donkey anti-Sheep IgG (H&L) - DyLight®650 Conjugated, min. cross-reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight® 650, which binds to Sheep IgG (H&L) in immunological assays.
DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Sheep IgG
DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
- Protocols
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