Donkey anti-Sheep IgG (H&L), Biotin conjugated
AS09 611 | Clonality: Polyclonal | Host: Donkey | Reactivity: Sheep IgG (H&L)
- Product Info
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Immunogen: Purified sheep IgG, whole molecule
Host: Donkey Clonality: Polyclonal Purity: Immunogen purified donkey IgG. Format: Liquid Quantity: 2 mg Storage: Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt. Tested applications: ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 2000-1: 20 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 2000-1: 20 000 (WB) - Reactivity
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Confirmed reactivity: Sheep IgG heavy and light chains (H&L) Predicted reactivity: Sheep IgG Heavy and Light chains (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)
biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Additional information (application): No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. - Background
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Background: Donkey anti-sheep IgG is a secondary antibody conjugated to biotin which binds to sheep IgG immunoglobulins in immunological assays.
- Protocols
- Agrisera Western Blot Guide
- Reviews:
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