Anti-DOG1 alpha splice variant

Name: Anti-DOG1 alpha splice variant
SKU: AS15 3032
Availability: OutOfStock
Rating: 5 (1 reviews)

Product no: AS15 3032

AS15 3032 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Replaced by AS24 5008

DATA SHEET IN PDF

Delivery: Replaced by another product

Product Info

Immunogen:	Synthetic peptide, conjugated to KLH derived from a sequence of Arabidopsis thaliana DOG1, UniProt:A0SVK0, TAIR: AT5G45830
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 2000 (WB)
Expected \| apparent MW:	32.4 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Brachypodium distachyon, Brassica napus, Capsella rubella, Lepidium papillosum, Sisymbrium officinale Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Background

Background:

DOG1 alpha splice variant (Delay Of Germination 1) is involved in the control of seed dormancy.

Alternative names: ATDOG1, DELAY OF GERMINATION 1, DOG1, GAAS5, GERMINATION ABILITY AFTER STORAGE 5, GLUCOSE SENSING QTL 5, GSQ5.

Product Citations

Selected references:

Bryant et al. (2019). Basic LEUCINE ZIPPER TRANSCRIPTION FACTOR67 Transactivates DELAY OF GERMINATION1 to Establish Primary Seed Dormancy in Arabidopsis. Plant Cell. 2019 Jun;31(6):1276-1288. doi: 10.1105/tpc.18.00892
Cyrek et al. (2016). Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1. Plant Physiol. 2016 Feb;170(2):947-55. doi: 10.1104/pp.15.01483.

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Number of reviews: (1)

Anna Kulik | 2023-12-28

0 | 0

Antibodies work nicely for Western Blot in seeds. We were able to easly detect DOG1 protein when loading 35 ug of total protein extract per sample. No band was detected in dog1 KO mutants.