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Anti-Clathrin heavy-chain 1,2, ALP-conjugated (40 ”g)
AS10 690-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
- Product Info
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Immunogen: KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP. Format: Liquid Quantity: 40 ”g Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Tested applications: Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB) Recommended dilution: 1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB) Expected | apparent MW: 193 | 170 kDa (Arabidopsis thaliana)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum Predicted reactivity: Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: Nicotiana benthamiana
- Additional Information
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Additional information (application): Please, note that fresh samples will provide better restuls (see image below) - Background
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Background: Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
- Product Citations
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Selected references: Gao et al. (2019). The Arabidopsis receptor kinase STRUBBELIG undergoes clathrin-dependent endocytosis. J Exp Bot. 2019 Apr 25. pii: erz190. doi: 10.1093/jxb/erz190.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
AS10 680 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardii, E. gracilis, H. vulgare, O. sativa, Setaria italica, Zea mays
AS10 681 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Medicago sativa
AS10 690 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum