Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - DyLight® 650 conjugated (40 µg)

Product no: AS16 3690-DL650
AS16 3690-DL650 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cas9 from Streptococcus thermophilus
490 €
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DATA SHEET IN PDF
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6
    (peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.
    Format: Liquid in PBS pH 7,4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user.
    Expected | apparent MW: Depends upon a MW of a protein which is a fusion partner.
  • Reactivity
  • Confirmed reactivity: Staphylococcus aureus
    Predicted reactivity: Staphylococcus pyogenes UniProt: Q99ZW2
  • Additional Information
  • Additional information: DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
  • Background
  • Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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