Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - DyLight® 594 conjugated (40 µg)
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6
(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.
Format: Liquid in PBS pH 7,4. Quantity: 40 µg Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use. Tested applications: Immunofluorescence (IF), Western blot (WB) Recommended dilution: To be determined by end user. Expected | apparent MW: Depends upon a MW of a protein which is a fusion partner. - Reactivity
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Confirmed reactivity: Staphylococcus aureus Predicted reactivity: Staphylococcus pyogenes UniProt: Q99ZW2 - Additional Information
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Additional information: DyLight® 594 has Amax = 593 nm, Emax = 618 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries. - Background
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Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection
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