Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - DyLight® 488 conjugated (40 µg)

Name: Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - DyLight® 488 conjugated (40 µg)
SKU: AS16 3690-DL488
Price: 490 EUR
Availability: InStock

Product no: AS16 3690-DL488

AS16 3690-DL488 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cas9 from Streptococcus thermophilus

490 €

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DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6 (peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 488.
Format:	Liquid in PBS pH 7,4.
Quantity:	40 µg
Storage:	Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
Tested applications:	Immunofluorescence (IF), Western blot (WB)
Recommended dilution:	To be determined by end user.
Expected \| apparent MW:	Depends upon a MW of a protein which is a fusion partner.

Reactivity
Confirmed reactivity: Staphylococcus aureus

Predicted reactivity: Staphylococcus pyogenes UniProt: Q99ZW2
Additional Information
Additional information: DyLight® 488 Amax = 493 nm, Emax = 519 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
Background

Background:

Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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