IKP

Anti-Cas9 | CRISPR-associated endonuclease 9 - HRP conjugated, (monoclonal)

Name: Anti-Cas9 | CRISPR-associated endonuclease 9 - HRP conjugated, (monoclonal)
Brand: IKP
SKU: AS22 4780
Price: 573 EUR
Availability: InStock

Product no: AS22 4780

AS22 4780 | Clonality: Monoclonal | Host: Mouse | Reactivity: Cas9 from Streptococcus pyogenes

573 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Data sheet
Product Info

Immunogen:	Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
Sub class:	IgG1k
Host:	Mouse
Clonality:	Monoclonal
Purity:	IgG1k Protein G purified in Guardian Peroxidase Conjugate Stabilizer (Thermo Scientific cat. No.37568), conjugated to HRP (Horseradish peroxidase).
Format:	Liquid
Quantity:	500 µl
Storage:	Store at 4°C; Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications:	Western blot (WB)
Recommended dilution:	1: 100 (WB)
Expected \| apparent MW:	Depends upon the MW of the protein which is the fusion partner.

Reactivity
Confirmed reactivity: Cas9 from Streptococcus pyogenes
Application Examples

Western blot using anti CRISPR, HRP conjugated monoclonal antibodies

Protein extracts from HeLa cells were transfected with a FLAG-tagged Cas9.
Primary anti-CRISPR/Cas9 antibodies were titrated from 1: 10 to 1: 100. The arrow to the right shows the position of the FLAG-tagged Cas9 protein.

Background

Background:

Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.

Protocols
Antibody protocols
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