IKP

Anti-Cas9 | CRISPR-associated endonuclease 9 - HRP conjugated, (monoclonal)

Product no: AS22 4780

AS22 4780  |  Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: Cas9 from Streptococcus pyogenes

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  • Data sheet

  • Product Info
  • Immunogen: Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
    Sub class: IgG1k
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG1k Protein G purified in Guardian Peroxidase Conjugate Stabilizer (Thermo Scientific cat. No.37568), conjugated to HRP (Horseradish peroxidase).
    Format: Liquid
    Quantity: 500 µl
    Storage: Store at 4°C;  Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1: 100 (WB)
    Expected | apparent MW: Depends upon the MW of the protein which is the fusion partner.
  • Reactivity
  • Confirmed reactivity: Cas9 from Streptococcus pyogenes
  • Application Examples
  • Western blot using anti CRISPR, HRP conjugated monoclonal antibodies

    Protein extracts from HeLa cells were transfected with a FLAG-tagged Cas9.
    Primary anti-CRISPR/Cas9 antibodies were titrated from 1: 10 to 1: 100. The arrow to the right shows the position of the FLAG-tagged Cas9 protein. 

  • Background
  • Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.
  • Protocols
  • Antibody protocols
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