Anti-BirA (mutated/TurboID) - DyLight® 650 conjugated (40 µg)

Product no: AS20 4440-DL650
AS20 4440-DL650  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
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  • Product Info
  • Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 650.

    Format: Liquid in PBS pH 7,4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user.
    Expected | apparent MW: Depends upon fusion partner
  • Reactivity
  • Confirmed reactivity: BirA (mutated/TurboID)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
  • Additional Information
  • Additional information: DyLight® 650 has Amax = 652 nm, Emax = 672 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
  • Background
  • Background: Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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