Anti-BirA (mutated/TurboID) - DyLight® 594 conjugated (40 µg)

Name: Anti-BirA (mutated/TurboID) - DyLight® 594 conjugated (40 µg)
SKU: AS20 4440-DL594
Price: 490 EUR
Availability: InStock

Product no: AS20 4440-DL594

AS20 4440-DL594 | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms

490 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.
Format:	Liquid in PBS pH 7,4.
Quantity:	40 µg
Storage:	Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
Tested applications:	Immunofluorescence (IF), Western blot (WB)
Recommended dilution:	To be determined by end user.
Expected \| apparent MW:	Depends upon fusion partner

Reactivity
Confirmed reactivity: BirA (mutated/TurboID)

Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
Additional Information
Additional information: DyLight® 594 has Amax = 593 nm, Emax = 618 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
Background

Background:

Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Agrisera Educational Posters Collection
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