Anti-Biotin (Monoclonal, clone 15D9)

Product no: AS22 4752
AS22 4752 | Clonality: Monoclonal  | Host: Mouse | Reactivity: Biotin
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  • Product Info
  • Sub class: IgG2b / Kappa
    Immunogen: Biotin
    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified on Protein A to a total immunoglobulin fraction, in Tris 0.1M, glycine 0.1M and 2 % sucrose.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: Reconstitute in distilled water before use.
    Storage: Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1:5000 - 1:10 000 (ELISA), 1: 200 - 1: 2000 (IHC), 1: 1000 - 1: 5000 (WB)
    Expected | apparent MW: Depends upon fusion partner
  • Reactivity
  • Confirmed reactivity: Antibody detects free biotin and biotin bound on a carrier protein.
  • Background
  • Background: Biotin has a high affinity to avidin or streptavidin and is there used as a common tag.
  • Protocols
  • Antibody protocols
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