Anti-Biotin (Monoclonal, clone 15D9)
- Product Info
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Sub class: IgG2b / Kappa Immunogen: Biotin Host: Mouse Clonality: Monoclonal Purity: Purified on Protein A to a total immunoglobulin fraction, in Tris 0.1M, glycine 0.1M and 2 % sucrose. Format: Lyophilized Quantity: 100 µg Reconstitution: Reconstitute in distilled water before use. Storage: Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1:5000 - 1:10 000 (ELISA), 1: 200 - 1: 2000 (IHC), 1: 1000 - 1: 5000 (WB) Expected | apparent MW: Depends upon fusion partner - Reactivity
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Confirmed reactivity: Antibody detects free biotin and biotin bound on a carrier protein. - Background
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Background: Biotin has a high affinity to avidin or streptavidin and is there used as a common tag. - Protocols
- Antibody protocols
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Accessories
AS10 666 | Clonality: Polyclonal | Host: Goat | Reactivity: free biotin, biotin conjugated to KLH or BSA