Goat anti-biotin, HRP conjugated
- Product Info
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Immunogen: Biotin Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. Recommended dilution: This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, - Additional Information
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Additional information: Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml, Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,1 % (v/v) Kathon CG as preservative of bacterial growth - Background
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Background: Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. - Protocols
- Antibody protocols
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Accessories
AS10 666 | Clonality: Polyclonal | Host: Goat | Reactivity: free biotin, biotin conjugated to KLH or BSA