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Anti-b-PE | Phycoerythrobilin
AS08 279 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Red algae, cyanobacteria and most cryptomonads
- Product Info
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Immunogen: native purified b-phycoerythrin of Porphyridium cruentum (protein with attached phycobilisomes)
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 2000 (ELISA), 1 : 1000 - 5000 (WB) Expected | apparent MW: 17-21 kDa
- Reactivity
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Confirmed reactivity: Porphyridium cruentum Predicted reactivity: Algae (red), Cyanobacteria, Cryptomonads
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Background
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Background: In phycobilisomes, the light-harvesting antennae of red algae, b-phycoerythrin consists of alpha and beta chains that contain phycoerythrobilin chromophores (an open-chain tetrapyrolle, red colored). It is found in chloroplasts of many red algae, and is closely related to the PEs of cyanobacteria and cryptomonads.
- Product Citations
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Selected references: Usuldin et al. (2017). Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach. ournal of Applied Phycology, August 2017, Volume 29, Issue 4, pp 1989–2001. (Kappaphycus alvarezii)
Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt & Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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Accessories
AS08 277 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Red algae, cyanobacteria
AS08 278 | Clonality:Polyclonal | Host:Rabbit | Reactivity: Read algae, cyanobacteria
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)