Anti-ATG16 | Autophagy-related protein 16

Product no: AS19 4280

AS19 4280  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: N-terminal part of of ATG16 of Arabidopsis thaliana UniProt: Q6NNP0, TAIR: At5g50230
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 56.4 | >70 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application examples:

    Reactant: Aponogeton madagascariensis

    Application: Western Blotting

    Pudmed ID: 36795694

    Journal: PLoS One

    Figure Number: 2C

    Published Date: 2023-02-17

    First Author: Rowarth, N. M.

    Impact Factor: 3.582

    Open Publication

    Detection of lace plant Atg16 in lace plant leaves.RNA and protein were extracted and probed for Atg16 from imperforate (I), pre-perforation (P), window (W), or mature (M) lace plant leaf stages (A-D) or only window and mature leaves from plants treated with either 5 μM rapamycin (Rap), 1 μM wortmannin (Wrt), and 1 μM concanamycin A (ConA) compared to DMSO control (E-H). (A and E) The mean levels of Atg16 mRNA in different lace plant leaf stages were determined by qRT-PCR and normalized to lace plant α-tubulin levels. Protein extracts and a molecular protein standard (L) were resolved by SDS–polyacrylamide gels and blotted to nitrocellulose membranes. (B and F) Membranes and protein lanes were stained with Ponceau-S to serve as loading control before subsequent detection of the presence or absence of ~56 kDa sized Atg16 protein bands with anti-Atg16 antibody (C and G). (D and H) Immunoreactive protein bands were quantitated, and the ratio of Atg16 band intensity to the Ponceau lane signal was averaged. The experiments were performed in triplicate. Means not sharing any letter are significantly different. One-way ANOVA (A, D and H), or Two-way ANOVA, Tukey test (E, P < 0.05; n = 3). Error bars represent the SE.


    Reactant: Aponogeton madagascariensis

    Application: Western Blotting

    Pudmed ID: 36795694

    Journal: PLoS One

    Figure Number: 2G

    Published Date: 2023-02-17

    First Author: Rowarth, N. M.

    Impact Factor: 3.582

    Open Publication

    Detection of lace plant Atg16 in lace plant leaves.RNA and protein were extracted and probed for Atg16 from imperforate (I), pre-perforation (P), window (W), or mature (M) lace plant leaf stages (A-D) or only window and mature leaves from plants treated with either 5 μM rapamycin (Rap), 1 μM wortmannin (Wrt), and 1 μM concanamycin A (ConA) compared to DMSO control (E-H). (A and E) The mean levels of Atg16 mRNA in different lace plant leaf stages were determined by qRT-PCR and normalized to lace plant α-tubulin levels. Protein extracts and a molecular protein standard (L) were resolved by SDS–polyacrylamide gels and blotted to nitrocellulose membranes. (B and F) Membranes and protein lanes were stained with Ponceau-S to serve as loading control before subsequent detection of the presence or absence of ~56 kDa sized Atg16 protein bands with anti-Atg16 antibody (C and G). (D and H) Immunoreactive protein bands were quantitated, and the ratio of Atg16 band intensity to the Ponceau lane signal was averaged. The experiments were performed in triplicate. Means not sharing any letter are significantly different. One-way ANOVA (A, D and H), or Two-way ANOVA, Tukey test (E, P < 0.05; n = 3). Error bars represent the SE.

  • Background
  • Background: ATG16 (Autophagy-related protein 16) may play a role in autophagy process.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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