Anti-ATG12b | Autophagy-related protein 12b

Name: Anti-ATG12b | Autophagy-related protein 12b
SKU: AS19 4278
Price: 319 EUR
Availability: InStock
Rating: 3 (1 reviews)

Product no: AS19 4278

AS19 4278 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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DATA SHEET IN PDF

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Product Info

Immunogen:	Recombinant, full-length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	10.4 \| 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy-deficient mutants

Reactivity
Confirmed reactivity: Arabidopsis thaliana

Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information
Additional information (application): This antibody may also recognise ATG12a; in wild-type, ATG12 is also conjugated to ATG5
Background

Background:

ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation.

Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.

Product Citations

Selected references:

Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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Number of reviews: (1)

Jose Julian | 2021-05-18

0 | 0

We were able to detect the correct protein thanks to this antibody but it also presented quite abundant background.