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Anti-RFP | Red flourescent protein (mRFP, mCherry, tdTomato, mScarlet)
AS24 5006 | Clonality: Polyclonal | Host: Rabbit | Reactivity: mRFP/mCherry/tdTomato/mScarlet tagged proteins
- Product Info
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Immunogen: Recombinant protein produced in E.coli corresponding to full lenght mRFP. The sequence used for immunization is also found in other red flourescent proteins like: mCherry/tdTomato/mScarlet and others.
Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Immunohistochemistry (IHC) Recommended dilution: 1: 200 (IHC) Expected | apparent MW: Depends upon fusion partner - Reactivity
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Confirmed reactivity: RFP Predicted reactivity: mRFP, mCherry, tdTomato, mScarlet Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Type of material: Mouse intestinal tissue (FFPE tissue), overexpressing mScarlet tagged protein
Fixation: formaldehyde
Hydrophilization: n/a
Cell wall digestion: n/a
Membrane permeabilization: n/a
Antigen retrieval: in citrate buffer
Blocking buffer: Superblock plus (Thermoscientific 37580) Washing buffer: PBS
Primary antibody dilution and incubation time: 1:200/ 1 h incubation
Secondary antibody: Vector labs, BA-1000-Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated 1:200
Co-staining of the nucleus: HaematoxylinCourtesy of Dr. Hayley Belnoue-Davis, Centre for Human Genetics, University of Oxford, United Kingdom
- Background
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Background: Red fluorescent protein (RFP) once excited is fluorescing red-orange light. The mass of RFP is approximately 25.9 kDa and its excitation maximum is 558 nm and emission maximum is 583 nm. Improved variants of RFP include so called mFruits variants: mCherry, mOrange, mRaspberry. - Product Citations
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Selected references: 38221900 - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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