Agrisera ECL set (Bright/SuperBright) (100 ml)

Set products

Agrisera ECL SuperBright: 2 x 50 ml, two-component, ready-to-use solutions. Enough for 50 midi blots (6.8 x 8.1 cm).
Agrisera ECL Bright: 2 x 50 ml, two-component, ready-to-use solutions. Enough for 50 midi blots (6.8 x 8.1 cm).

This set combines reagents with low pico to mid femtogram and extreme low femtogram detection range combined with low background and superior signal-to-noise ratios

Applications

Western blot (WB)

Storage

Store at 2°C to 8°C. Mixed working reagent is stable for several days at room temperature or at 4°C. Exceptional lot-to-lot consistency.


User Instructions

• Store reagents A and B in the darkness at 4-8°C.
• Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.
• Prepare your membrane prior addition of chemiluminescent substrate, by a wash with the buffer used in your protocol (PBS or TBS or TBST-T). This will allow to remove any background prior to substrate contact.
• Optimal visualization is obtained up to 20 minutes after substrate contact. Incubation for 2-5 minutes is usually optimal.
• Remove excess substrate by filter paper.
• Cover blot with clear plastic wrap or sheet protector and expose either with x-ray film or CCD camera.

In some cases Tween can quench the reaction. For best results clean containers and high quality water has to be used.

Protocols

Antibody protocols
Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts

HS code for this product is: 3822.00.0002.