IKP

Agrisera ECL set (Bright/SuperBright) (100 ml)

Product no: AS16 ECL-S-N-100
AS16 ECL-S-N-100 | Low pico to mid femtogram and extreme low femtogram detection
177.60
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Delivery 3-6 business days
Delivery:  3-6 business days
  • Data sheet
  • Set products

    Agrisera ECL SuperBright: 2 x 50 ml, two-component, ready-to-use solutions. Enough for 50 midi blots (6.8 x 8.1 cm).
    Agrisera ECL Bright
    : 2 x 50 ml, two-component, ready-to-use solutions. Enough for 50 midi blots (6.8 x 8.1 cm).

    This set combines reagents with low pico to mid femtogram and extreme low femtogram detection range combined with low background and superior signal-to-noise ratios

    Applications

    Western blot (WB)


    Storage

    Store at 2°C to 8°C. Mixed working reagent is stable for several days at room temperature or at 4°C. Exceptional lot-to-lot consistency.


    User Instructions

    • Store reagents A and B in the darkness at 4-8°C.
    • Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.
    • Prepare your membrane prior addition of chemiluminescent substrate, by a wash with the buffer used in your protocol (PBS or TBS or TBST-T). This will allow to remove any background prior to substrate contact.
    • Optimal visualization is obtained up to 20 minutes after substrate contact. Incubation for 2-5 minutes is usually optimal.
    • Remove excess substrate by filter paper.
    • Cover blot with clear plastic wrap or sheet protector and expose either with x-ray film or CCD camera.

    In some cases Tween can quench the reaction. For best results clean containers and high quality water has to be used.

    Protocols

    Antibody protocols
    Agrisera Western Blot protocol and video tutorials
    Protocols to work with plant and algal protein extracts

    HS code for this product is: 3822.00.0002.

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