Anti-AGO1 | Argonaute 1, DyLight® 488 conjugated (40 µg)

Product no: AS09 527-DL488
AS09 527-DL488 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana
490 €
Add to cart
DATA SHEET IN PDF
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
    Host: Rabbit
    Clonality: Polyclonal
    Purity:

    Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 488.
    Format: Liquid in PBS pH 7.4.
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
    Tested applications: Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: To be determined by end user.
    Expected | apparent MW: 116.4 | 130 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Nicotiana benthamiana
    Predicted reactivity: Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

    Species of your interest not listed? Contact us
    Not reactive in: Chlamydomonas reinhardtii
  • Additional Information
  • Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U,TCA acetone total protein precipitation method.

    DyLight® 488 Amax = 493 nm, Emax = 519 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
    Additional information (application): AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
  • Background
  • Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi).
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Save
    Leave a review
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

Anti-AGO1 | Argonaute 1 (40 µg, ALP-conjugated)

AS09 527-ALP  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

490 €
Add to cart
Info
Anti-AGO1 | Argonaute 1 (40 µg, Biotin conjugated)

AS09 527B  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

490 €
Add to cart
Info
AGO, Argonaute set | 4 antibodies
AS18 4248 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

This set contains:
- 4 Anti-AGO antibodies of your choice, chosen from a drop down menu below
- Matching secondary antibody, HRP conjugated, min. 1: 25 000, 1h/RT (AS09 602 - trial)

1167 €
Add to cartInfo
Anti-HYL1 | Hyponastic leave phenotype ds-RNA binding protein

AS06 136  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
Close
Login / Retailer
Register
Forgot password?