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Anti-AO | L-ascorbate oxidase
Product no: AS19 4337
AS19 4337 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cucurbita maxima
241
€
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- Product Info
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Immunogen: Recombinant Cucurbita maxima L-ascorbate oxidase protein, amino acids: 31-579. UniProt: P24792
Host: Rabbit Clonality: Polyclonal Purity: >95%, Protein G purified to a total immunoglobulin G fraction. Format: Liquid Quantity: 50 µg Storage: Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 1000 - 1: 5000 (WB) Expected | apparent MW: 65 kDa - Reactivity
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Confirmed reactivity: Cucurbita maxima Predicted reactivity: Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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application example
40, 20, 10 and 5 µg of Cucurbita maxima recombinant AO were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk in PBS-T for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions. - Additional Information
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Additional information: Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4
Reactivity of this antibody on endogenous material remains to be determined.Additional information (application): Reactivity of this antibody on endogenous material remains to be determined - Background
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Background: L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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