Set of 4 Chlamydomonas anti-Lhc antibodies

This set contains the following antibodies:

AS01 003  Lhcb2 | LHCII type II chlorophyll a/b-binding protein (50 µl)

AS06 117  Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas (100 µl)

AS09 407  Lhcb5 | CP26 (Lhcb5) homolog, Chlamydomonas (50 µl)

AS09 408  Lhcbm5 | chlorophyll a-b binding protein of LHCII (50 µl)

• Product Info

• Immunogen:	Lhcb2 - BSA-conjugated peptide Lhcb4 - KLH-conjugated synthetic peptide derived from Lhcb4 (CP29, Lhcbm4) protein sequence from Chlamydomonas reinhardtii (Q93WD2) Lhcb5 - KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence (Q9FEK6) Lhcbm5 - SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
  Host:	Rabbit
  Clonality:	Polyclonal
  Purity:	Total IgG. Protein G purified in PBS pH 7.4. (Lhcb2), serum (Lhcb4, Lhcb5, Lhcbm5).
  Format:	Lyophilized
  Quantity:	50 µl (Lhcb2, Lhcb5, Lhcbm5), 100 µl (Lhcb4)
  Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  Tested applications:	Western blot (WB)
  Recommended dilution:	1 : 5000 (Lhcb2, Lhcb5, Lhcbm5) (WB) 1 : 10 000 (Lhcb4) (WB)
  Expected | apparent MW:	25 kDa (Lhcb2) 29 kDa (Lhcb4) 29 | 30 kDa (Lhcbm5) 30 | 26 kDa (Lhcb5)

• Reactivity

• Confirmed reactivity:	Chlamydomonas reinhardtii
  Predicted reactivity:	Algae (Lhcb2), Angiosperms, Dictos, Gymnosperms, Mosses
  Not reactive in:	Lhcb4 - higher plants (use AS04 045 for those organsims), algae, cyanobacteria

• Background

• Background:

  The outer light-harvesting antenna of the photosystems (PSI and PSII) of the green unicellular alga Chlamydomonas reinhardtii is composed of pigment-binding proteins belonging to the Lhc family highly conserved in photosynthetic eukaryotes. Some of the Lhc-subtypes of Chlamydomonas share sufficient high similarity with the respective functional homologs in plants to allow specific detection with antisera generated against conserved peptide domains from plant Lhc-proteins.

• Product Citations

• Selected references:

  Lhcb2 Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446. Lhcb4 Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457. Lhcb5 and Lhcbm5 Takahashi et al. (2006). Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii. PNAS 103:477-482