Anti-ATG1a | Serine/threonine-protein kinase ATG1a

Product no: AS19 4274

AS19 4274  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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319 €
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DATA SHEET IN PDF
Delivery:  3-6 business days
  • Product Info
  • Immunogen: Full-length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana protein sequence UniProt: Q94C95, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece
    Host:

    Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 µl
    Reconstitution: For reconstitution add 50 µl, of sterile water
    Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 69.6 | 70 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Noccaea caerulescens

    Species of your interest not listed? Contact us
    Not reactive in: Oryza sativa
  • Additional Information
  • Additional information (application): Antibody may also recognise ATG1b and ATG1c; does not recognise ATG1t, Suttangkakul et al. (2011).
  • Background
  • Background: ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status.

    Alternative names: Autophagy-related protein 1a, AtAPG1a.
  • Product Citations
  • Selected references: Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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